Just to get Going

Any experiment worth doing one time is worth doing twice. Unless your having fun, or screening, or doing crazy pilot stuff one day out of the week. Some kid comes in and says the kit doesnt work. oh man the kit is for another organism. Hey, make a version for us, will you? Should be easy. Ok look I made that reagent but oops it doesnt work now what to I do? I say that you have to make sure you learned something about how it didnt work. Three goes on something. Thats error bars.

I spent time last week looking for a reagent worrying about whether it was rna-free. I neglected to remember that there are aloquots prewieghed by myself, hidden in my supplies. Hidden because I have tons of crap everywhere. Totally. Ran an experiment unrelated last week, considering the tow things I want out of the purification. Go for the real goal or check things out a little. Well see if you have any freaking expression first how about that. Do these reagents even work? This week is time for sequencing and two new experiments. Set time to set up the reagents, with check marks. Set up the work space. Post the protocal for check marks. Here we go academic year 09. I am a fifth year, and I am far less competent than I should be.

I have been saying that for years and now I am thinking that I have to actually give up a physical peice of time to work towards that goal. I think about changing things, and generally I continue to do the same thing that I am doing. Its a matter of time and intent. Even if I suddenly have time I do not really get the job done, even if I try, because of lack of intent. I am judging myself by judging other people, filing up the mental space of the day with short bursts of hate based on things I see. Things I think about. All my girlfriends have suffered from this symptom of mine because they each spend more time with me than others.